Osteoclast differentiation by human osteoblastic cell line SaOS-2 primed with bacterial lipid A
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چکیده
منابع مشابه
Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties.
This study examines the osteoblastic properties of the established human osteosarcoma cell line Saos-2. Saos-2 cells inoculated into diffusion chambers, which were implanted i.p. into nude mice, produced mineralized matrix in 4 of 6 chambers at 8 weeks. In 5 of 6 chambers there was a strong positive alkaline phosphatase reaction. In culture the alkaline phosphatase levels increased with time an...
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In an attempt to study the roles of G-protein stimulatory subunit α (Gsα) in osteoblasts, we introduced an expression vector encoding Gsα into human osteoblastic cell line SaOS-2, and established the clones stably overexpressing Gsα (SaOS-2-Gsα). In SaOS-2-Gsα, the intracellular content of cyclic AMP (cAMP) was increased compared with the parental SaOS-2 cells. In addition, when treated with PT...
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ژورنال
عنوان ژورنال: FEMS Immunology & Medical Microbiology
سال: 2003
ISSN: 0928-8244,1574-695X
DOI: 10.1016/s0928-8244(03)00111-1